Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Fe4-S4 cluster | active enzyme contains an iron-sulfur cluster | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
the recombinant yeast mitochondrial aconitase is expressed in Escherichia coli as soluble, biologically active enzyme | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using column chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
citrate | - |
Saccharomyces cerevisiae | cis-aconitate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aconitase | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
physiological function | aconitase is destabilized in the absence of the Fe4S4 cluster. Apo-form has higher surface hydrophobicity than the holo-form. The lower ground state stability and higher solvent exposed hydrophobic surface of the apo-form makes it aggregation prone. Binding of apo-aconitase to GroEL (molecular chaperone) not only rescues it from the aggregation, but also assists in the final stage of maturation by orienting the cluster insertion site of GroEL bound apo-protein | Saccharomyces cerevisiae |